Single-cell RNA sequencing of Tocilizumab-treated peripheral blood mononuclear cells

microbiologica

The RNase J-Based mostly RNA Degradosome Is Compartmentalized within the Gastric Pathogen Helicobacter pylori

Posttranscriptional regulation is a serious degree of gene expression management in any cell. In micro organism, multiprotein machines known as RNA degradosomes are central for RNA processing and degradation, and a few had been reported to be compartmentalized inside these organelleless cells. The minimal RNA degradosome of the essential gastric pathogen Helicobacter pylori consists of the important ribonuclease RNase J and RhpA, its sole DEAD field RNA helicase, and performs a serious position within the regulation of mRNA decay and adaptation to gastric colonization.

Right here, the subcellular localization of the H. pylori RNA degradosome was investigated utilizing mobile fractionation and each confocal and superresolution microscopy. 

We established that RNase J and RhpA are peripheral internal membrane proteins and that this affiliation was mediated neither by ribosomes nor by RNA nor by the RNase Y membrane protein. In dwell H. pylori cells, we noticed that fluorescent RNase J and RhpA protein fusions assemble into nonpolar foci. We recognized components that regulate the formation of those foci with out affecting the degradosome membrane affiliation.  

Flotillin, a bacterial membrane scaffolding protein, and free RNA promote focus formation in H. pylori Lastly, RNase J-GFP (RNase J-green fluorescent protein) molecules and foci in cells had been quantified by three-dimensional (3D) single-molecule fluorescence localization microscopy. 

The quantity and dimension of the RNase J foci had been discovered to be scaled with progress section and cell quantity as beforehand reported for eukaryotic ribonucleoprotein granules. In conclusion, we suggest that membrane compartmentalization and the regulated clustering of RNase J-based degradosome hubs symbolize essential ranges of management of their exercise and specificity.

 IMPORTANCE Helicobacter pylori is a bacterial pathogen that chronically colonizes the abdomen of half of the human inhabitants worldwide. An infection by H. pylori can result in the event of gastric pathologies akin to ulcers and adenocarcinoma, which causes as much as 800,000 deaths on this planet every year.

 Persistent colonization by H. pylori depends on regulation of the expression of adaptation-related genes. One main degree of such management is posttranscriptional regulation, which, in H. pylori, largely depends on a multiprotein molecular machine, an RNA degradosome, that we beforehand found. On this research, we established that the 2 protein companions of this machine are related to the membrane of H. pylori Utilizing cutting-edge microscopy, we confirmed that these complexes assemble into hubs whose formation is regulated by free RNA and scaled with bacterial dimension and progress section. Organelleless mobile compartmentalization of molecular machines into hubs emerges as an essential regulatory degree in micro organism.  

microbiologica
microbiologica

PEG3 Antibody

E043302 100μg/100μl
EUR 255
Description: Available in various conjugation types.

PEG3 Antibody

E11-11667C 100μg
EUR 225
Description: Available in various conjugation types.

PEG3 Antibody

E18-9152-1 50ug/50ul
EUR 145
Description: Available in various conjugation types.

PEG3 Antibody

E18-9152-2 100ug/100ul
EUR 225
Description: Available in various conjugation types.

PEG3 Antibody

CSB-PA022042- each
EUR 402
Description: A polyclonal antibody against PEG3. Recognizes PEG3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

PEG3 Antibody

CSB-PA022042-100ul 100ul
EUR 379.2
Description: A polyclonal antibody against PEG3. Recognizes PEG3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000

PEG3 Antibody

DF13596 100ul
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PEG3 Antibody

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PEG3 Antibody

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Description: Rabbit polyclonal PEG3 antibody

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PEG3 Antibody

1-CSB-PA030033
  • Ask for price
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  • 100ug
  • 50ug
Description: A polyclonal antibody against PEG3. Recognizes PEG3 from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/10000

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PEG3 Antibody

C11667-100ul 100μl
EUR 217
Description: PEG3 Rabbit Polyclonal Antibody

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C11667-50ul 50μl
EUR 143.5
Description: PEG3 Rabbit Polyclonal Antibody

PEG3 Polyclonal Antibody

ABP55568-003ml 0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of PEG3 from Human. This PEG3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human PEG3 at AA range: 1010-1090

PEG3 Polyclonal Antibody

ABP55568-01ml 0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of PEG3 from Human. This PEG3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human PEG3 at AA range: 1010-1090

PEG3 Polyclonal Antibody

ABP55568-02ml 0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of PEG3 from Human. This PEG3 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human PEG3 at AA range: 1010-1090

PEG3 Polyclonal Antibody

E20-73659 100ug
EUR 225
Description: Available in various conjugation types.

PEG3 Polyclonal Antibody

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Description: Available in various conjugation types.

PEG3 Conjugated Antibody

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PEG3 Polyclonal Antibody

E-AB-90450-120uL 120uL
EUR 320
Description: Unconjugated

PEG3 Polyclonal Antibody

E-AB-90450-200uL 200uL
EUR 530
Description: Unconjugated

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Description: Unconjugated

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E-AB-90450-each each Ask for price
Description: Unconjugated

PEG3 Polyclonal Antibody

E-AB-19429-120uL 120uL
EUR 240
Description: Unconjugated

PEG3 Polyclonal Antibody

E-AB-19429-200uL 200uL
EUR 399
Description: Unconjugated

PEG3 Polyclonal Antibody

E-AB-19429-20uL 20uL
EUR 73
Description: Unconjugated

PEG3 Polyclonal Antibody

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EUR 143
Description: Unconjugated

PEG3 Polyclonal Antibody

BT-AP07052-100ul 100ul Ask for price
Description: In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID: 15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms.

PEG3 Polyclonal Antibody

BT-AP07052-20ul 20ul Ask for price
Description: In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID: 15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms.

PEG3 Polyclonal Antibody

BT-AP07052-50ul 50ul Ask for price
Description: In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID: 15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms.

PEG3 Polyclonal Antibody

E44H03013 100ul
EUR 255
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

PEG3 Polyclonal Antibody

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EUR 415

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PEG3 Polyclonal Antibody

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PEG3 Polyclonal Antibody

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PEG3 Polyclonal Antibody

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PEG3 Polyclonal Antibody

MBS8567499-01mLAF405L 0.1mL(AF405L)
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PEG3 Polyclonal Antibody

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PEG3 Polyclonal Antibody

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PEG3 Polyclonal Antibody

MBS8567499-01mLAF635 0.1mL(AF635)
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PEG3 Polyclonal Antibody

MBS8524640-01mg 0.1mg
EUR 305

PEG3 Polyclonal Antibody

MBS8524640-01mLAF405L 0.1mL(AF405L)
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PEG3 Polyclonal Antibody

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PEG3 Polyclonal Antibody

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PEG3 Polyclonal Antibody

MBS2561318-006mL 0.06mL
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PEG3 Polyclonal Antibody

MBS2561318-012mL 0.12mL
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PEG3 Polyclonal Antibody

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PEG3 Polyclonal Antibody

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EUR 260

PEG3 Polyclonal Antibody

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EUR 405

PEG3 Conjugated Antibody

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EUR 480

PEG3 Conjugated Antibody

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EUR 480

PEG3 Conjugated Antibody

MBS9444916-01mLAF488 0.1mL(AF488)
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PEG3 Conjugated Antibody

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EUR 480

PEG3 Conjugated Antibody

MBS9444916-01mLBiotin 0.1mL(Biotin)
EUR 480

PEG3 Polyclonal Antibody

RD219429A-120uL 120μL
EUR 360
Description: In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms.

PEG3 Polyclonal Antibody

RD219429A-200uL 200μL
EUR 630
Description: In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms.

PEG3 Polyclonal Antibody

RD219429A-20uL 20μL
EUR 109.5
Description: In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms.

PEG3 Polyclonal Antibody

RD219429A-60uL 60μL
EUR 214.5
Description: In human, ZIM2 and PEG3 are treated as two distinct genes though they share multiple 5' exons and a common promoter and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect their shared 5' exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. In contrast, in other mammals ZIM2 does not undergo imprinting and, in mouse, cow, and likely other mammals as well, the ZIM2 and PEG3 genes do not share exons. Human PEG3 protein belongs to the Kruppel C2H2-type zinc finger protein family. PEG3 may play a role in cell proliferation and p53-mediated apoptosis. PEG3 has also shown tumor suppressor activity and tumorigenesis in glioma and ovarian cells. Alternative splicing of this PEG3 gene results in multiple transcript variants encoding distinct isoforms.

PEG3 Polyclonal Antibody

UB-GEN-1895 100 ul
EUR 200

Rabbit polyclonal PEG3 antibody

TA326281 100 µl Ask for price

OAAJ11874-100UL - PEG3 Antibody

OAAJ11874-100UL 100uL
EUR 319

PEG3 Rabbit Polyclonal Antibody

53415 100ul
EUR 439

PEG3 Rabbit Polyclonal Antibody

ES6567-100ul 100ul
EUR 124
Description: A Rabbit Polyclonal antibody against PEG3 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

PEG3 Rabbit Polyclonal Antibody

ES6567-50ul 50ul
EUR 74
Description: A Rabbit Polyclonal antibody against PEG3 from Human. This antibody is tested and validated for WB, ELISA, WB, ELISA

PEG3 Rabbit Polyclonal Antibody

E10G05715 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

PEG3 Rabbit Polyclonal Antibody

E10G12586 100 μl
EUR 275
Description: Biotin-Conjugated, FITC-Conjugated , AF350 Conjugated , AF405M-Conjugated ,AF488-Conjugated, AF514-Conjugated ,AF532-Conjugated, AF555-Conjugated ,AF568-Conjugated , HRP-Conjugated, AF405S-Conjugated, AF405L-Conjugated , AF546-Conjugated, AF594-Conjugated , AF610-Conjugated, AF635-Conjugated , AF647-Conjugated , AF680-Conjugated , AF700-Conjugated , AF750-Conjugated , AF790-Conjugated , APC-Conjugated , PE-Conjugated , Cy3-Conjugated , Cy5-Conjugated , Cy5.5-Conjugated , Cy7-Conjugated Antibody

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MBS9464677-005mL 0.05mL
EUR 300

PEG3 Rabbit Polyclonal Antibody

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PEG3 Rabbit Polyclonal Antibody

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EUR 1610

PEG3 antibody - C-terminal region

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PEG3 antibody - C-terminal region

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EUR 1995

Paternally Expressed 3 (PEG3) Antibody

20-abx013786
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  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Paternally Expressed 3 (PEG3) Antibody

abx331093-100ul 100 ul
EUR 510

Paternally Expressed 3 (PEG3) Antibody

20-abx323448
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  • 100 ug
  • 50 ug

PEG3 Polyclonal Antibody Cy5.5 Conjugated

MBS9464202-01mL 0.1mL
EUR 595

PEG3 Polyclonal Antibody Cy5.5 Conjugated

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PEG3 mouse monoclonal antibody,clone OTI1B2

TA801958 100 µl Ask for price

PEG3 mouse monoclonal antibody,clone OTI1B2

TA801958S 30 µl Ask for price

ARP38756_P050-25UL - PEG3 Antibody - C-terminal region

ARP38756_P050-25UL 25ul
EUR 99

Paternally-Expressed Gene 3 Protein (PEG3) Antibody

abx323448-100g 100 µg
EUR 250

Paternally-Expressed Gene 3 Protein (PEG3) Antibody

abx323448-50g 50 µg
EUR 187.5

Paternally-Expressed Gene 3 Protein (PEG3) Antibody

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EUR 43.75

ARP38756_P050 - PEG3 antibody - C-terminal region (ARP38756_P050)

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Amine-PEG3-Lys(PEG3-N3)-PEG3-N3

HY-153672 10 mg
EUR 865.81
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PEG3 mouse monoclonal antibody, clone OTI1E10 (formerly 1E10)

TA801960 100 µl Ask for price

PEG3 mouse monoclonal antibody, clone OTI1E10 (formerly 1E10)

TA801960S 30 µl Ask for price

PEG3 mouse monoclonal antibody, clone OTI1F12 (formerly 1F12)

TA801961 100 µl Ask for price

PEG3 mouse monoclonal antibody, clone OTI1F12 (formerly 1F12)

TA801961S 30 µl Ask for price

Mal-PEG4-(PEG3-DBCO)-(PEG3-TCO)

HY-136084 1mg Ask for price
Description: Mal-PEG4-(PEG3-DBCO)-(PEG3-TCO) is a cleavable 10 unit PEG ADC linker used in the synthesis of antibody-drug conjugates (ADCs)[1]. Mal-PEG4-(PEG3-DBCO)-(PEG3-TCO) is a click chemistry reagent, it contains a TCO group that can undergo an inverse electron demand Diels-Alder reaction (iEDDA) with molecules containing Tetrazine groups.

N-(PEG3-acid)-N-bis(PEG3-amine)

T16245-10mg 10mg Ask for price
Description: N-(PEG3-acid)-N-bis(PEG3-amine)

N-(PEG3-acid)-N-bis(PEG3-amine)

T16245-1g 1g Ask for price
Description: N-(PEG3-acid)-N-bis(PEG3-amine)

N-(PEG3-acid)-N-bis(PEG3-amine)

T16245-1mg 1mg Ask for price
Description: N-(PEG3-acid)-N-bis(PEG3-amine)

N-(PEG3-acid)-N-bis(PEG3-amine)

T16245-50mg 50mg Ask for price
Description: N-(PEG3-acid)-N-bis(PEG3-amine)

N-(PEG3-acid)-N-bis(PEG3-amine)

T16245-5mg 5mg Ask for price
Description: N-(PEG3-acid)-N-bis(PEG3-amine)

N-(PEG3-acid)-N-bis(PEG3-amine)

HY-140246 Get quote Ask for price
Description: N-(PEG3-acid)-N-bis(PEG3-amine) is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1].

PEG3 siRNA

20-abx928229
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  • 15 nmol
  • 30 nmol

PEG3 siRNA

20-abx928230
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  • 15 nmol
  • 30 nmol

PEG3-Tos

572441 1.0g
EUR 570

PEG3 Peptide

MBS3229226-01mg 0.1mg
EUR 180

PEG3 Peptide

MBS3229226-5x01mg 5x0.1mg
EUR 730

PEG3-methylamine

T16457-10mg 10mg Ask for price
Description: PEG3-methylamine

PEG3-methylamine

T16457-1g 1g Ask for price
Description: PEG3-methylamine

PEG3-methylamine

T16457-1mg 1mg Ask for price
Description: PEG3-methylamine

PEG3-methylamine

T16457-50mg 50mg Ask for price
Description: PEG3-methylamine

PEG3-methylamine

T16457-5mg 5mg Ask for price
Description: PEG3-methylamine

PEG3-methylamine

HY-140161 100 mg
EUR 54.11
Description: PEG3-methylamine is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1].

N-(Azido-PEG3)-N-(PEG2-amine)-PEG3-acid

T16192-10mg 10mg Ask for price
Description: N-(Azido-PEG3)-N-(PEG2-amine)-PEG3-acid

N-(Azido-PEG3)-N-(PEG2-amine)-PEG3-acid

T16192-1g 1g Ask for price
Description: N-(Azido-PEG3)-N-(PEG2-amine)-PEG3-acid

N-(Azido-PEG3)-N-(PEG2-amine)-PEG3-acid

T16192-1mg 1mg Ask for price
Description: N-(Azido-PEG3)-N-(PEG2-amine)-PEG3-acid

N-(Azido-PEG3)-N-(PEG2-amine)-PEG3-acid

T16192-50mg 50mg Ask for price
Description: N-(Azido-PEG3)-N-(PEG2-amine)-PEG3-acid

N-(Azido-PEG3)-N-(PEG2-amine)-PEG3-acid

T16192-5mg 5mg Ask for price
Description: N-(Azido-PEG3)-N-(PEG2-amine)-PEG3-acid

N-(Azido-PEG3)-N-(PEG2-amine)-PEG3-acid

HY-140247 100 mg
EUR 595.25
Description: N-(Azido-PEG3)-N-(PEG2-amine)-PEG3-acid is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1]. N-(Azido-PEG3)-N-(PEG2-amine)-PEG3-acid is a click chemistry reagent, it contains an Azide group and can undergo copper-catalyzed azide-alkyne cycloaddition reaction (CuAAc) with molecules containing Alkyne groups. Strain-promoted alkyne-azide cycloaddition (SPAAC) can also occur with molecules containing DBCO or BCN groups.

Peg3 (untagged) - Mouse paternally expressed 3 (Peg3), (10ug)

MC224695 10 µg Ask for price

N-(Boc-PEG3)-N-bis(PEG3-acid)

T18403-10mg 10mg Ask for price
Description: N-(Boc-PEG3)-N-bis(PEG3-acid)

N-(Boc-PEG3)-N-bis(PEG3-acid)

T18403-1g 1g Ask for price
Description: N-(Boc-PEG3)-N-bis(PEG3-acid)

N-(Boc-PEG3)-N-bis(PEG3-acid)

T18403-1mg 1mg Ask for price
Description: N-(Boc-PEG3)-N-bis(PEG3-acid)

N-(Boc-PEG3)-N-bis(PEG3-acid)

T18403-50mg 50mg Ask for price
Description: N-(Boc-PEG3)-N-bis(PEG3-acid)

N-(Boc-PEG3)-N-bis(PEG3-acid)

T18403-5mg 5mg Ask for price
Description: N-(Boc-PEG3)-N-bis(PEG3-acid)

N-(Boc-PEG3)-N-bis(PEG3-acid)

HY-140536 100 mg
EUR 638.54
Description: N-(Boc-PEG3)-N-bis(PEG3-acid) is a PEG-based PROTAC linker that can be used in the synthesis of PROTACs[1].

N-(NHS-PEG3)-N-bis(PEG3-azide)

572830 100.0mg
EUR 530

N-(Azido-PEG3)-N-bis(PEG3-Boc)

T18379-10mg 10mg Ask for price
Description: N-(Azido-PEG3)-N-bis(PEG3-Boc)

N-(Azido-PEG3)-N-bis(PEG3-Boc)

T18379-1g 1g Ask for price
Description: N-(Azido-PEG3)-N-bis(PEG3-Boc)

N-(Azido-PEG3)-N-bis(PEG3-Boc)

T18379-1mg 1mg Ask for price
Description: N-(Azido-PEG3)-N-bis(PEG3-Boc)

N-(Azido-PEG3)-N-bis(PEG3-Boc)

T18379-50mg 50mg Ask for price
Description: N-(Azido-PEG3)-N-bis(PEG3-Boc)

N-(Azido-PEG3)-N-bis(PEG3-Boc)

T18379-5mg 5mg Ask for price
Description: N-(Azido-PEG3)-N-bis(PEG3-Boc)

N-(Amino-PEG3)-N-bis(PEG3-Boc)

T16171-10mg 10mg Ask for price
Description: N-(Amino-PEG3)-N-bis(PEG3-Boc)

N-(Amino-PEG3)-N-bis(PEG3-Boc)

T16171-1g 1g Ask for price
Description: N-(Amino-PEG3)-N-bis(PEG3-Boc)

Single-cell RNA sequencing of Tocilizumab-treated peripheral blood mononuclear cells as an in vitro mannequin of irritation

COVID-19 has posed a major risk to international well being. Early knowledge has revealed that IL-6, a key regulatory cytokine, performs an essential position within the cytokine storm of COVID-19. A number of trials are due to this fact wanting on the results of Tocilizumab, an IL-6 receptor antibody that inhibits IL-6 exercise, on remedy of COVID-19, with promising findings. 

As a part of a scientific trial wanting on the results of Tocilizumab remedy on kidney transplant recipients with subclinical rejection, we carried out single-cell RNA sequencing of evaluating stimulated PBMCs earlier than and after Tocilizumab remedy. We leveraged this knowledge to create an in vitro cytokine storm mannequin, to higher perceive the consequences of Tocilizumab within the presence of irritation. Tocilizumab-treated cells had lowered expression of inflammatory-mediated genes and biologic pathways, notably amongst monocytes. These outcomes help the speculation that Tocilizumab might hinder the cytokine storm of COVID-19, by way of an indication of biologic influence on the single-cell degree.   

Useful Characterization of a Putative RNA Demethylase ALKBH6 in Arabidopsis Development and Abiotic Stress Responses

RNA methylation and demethylation, which is mediated by RNA methyltransferases (known as “writers”) and demethylases (known as “erasers”), respectively, are rising as a key regulatory course of in plant growth and stress responses. Though a number of research have proven that AlkB homolog (ALKBH) proteins are potential RNA demethylases, the operate of most ALKBHs is but to be decided. 

 The Arabidopsis thaliana genome accommodates 13 genes encoding ALKBH proteins, the capabilities of that are largely unknown. On this research, we characterised the operate of a possible eraser protein, ALKBH6 (At4g20350), throughout seed germination and seedling progress in Arabidopsis below abiotic stresses. The seeds of T-DNA insertion alkbh6 knockdown mutants germinated quicker than the wild-type seeds below chilly, salt, or abscisic acid (ABA) remedy situations however not below dehydration stress situations. 

Though no variations in seedling and root progress had been noticed between the alkbh6 mutant and wild-type below regular situations, the alkbh6 mutant confirmed a a lot decrease survival fee than the wild-type below salt, drought, or warmth stress. Cotyledon greening of the alkbh6 mutants was a lot greater than that of the wild-type upon ABA utility.  Furthermore, the transcript ranges of ABA signaling-related genes, together with ABI3 and ABI4, had been down-regulated within the alkbh6 mutant in comparison with wild-type vegetation. Importantly, the ALKBH6 protein had a capability to bind to each m6A-labeled and m5C-labeled RNAs. Collectively, these outcomes point out that the potential eraser ALKBH6 performs essential roles in seed germination, seedling progress, and survival of Arabidopsis below abiotic stresses.  

GNAS Antibody

E10-30781T 100ul
EUR 225
Description: Available in various conjugation types.

GNAS Antibody

E19-7068 100μg/100μl
EUR 225
Description: Available in various conjugation types.

GNAS Antibody

E19-7388 100μg/100μl
EUR 225
Description: Available in various conjugation types.

GNAS Antibody

DF7068 200ul
EUR 420

GNAS Antibody

DF7068-100ul 100ul
EUR 280

GNAS Antibody

DF7068-200ul 200ul
EUR 350

GNAS Antibody

DF7388 200ul
EUR 420

GNAS Antibody

DF7388-100ul 100ul
EUR 280

GNAS Antibody

DF7388-200ul 200ul
EUR 350

GNAS Antibody

E92732 100ul
EUR 255
Description: Available in various conjugation types.

GNAS Antibody

E91416 100μg
EUR 255
Description: Available in various conjugation types.

GNAS Antibody

E95546 100μg
EUR 255
Description: Available in various conjugation types.

GNAS Antibody

CAC11323-100ug 100ug
EUR 314

GNAS Antibody

CAC11323-50ug 50ug
EUR 199.2

GNAS Antibody

CAC10438-100ug 100ug
EUR 314

GNAS Antibody

CAC10438-50ug 50ug
EUR 199.2

GNAS antibody

70R-12940 100 ul
EUR 550
Description: Affinity purified Rabbit polyclonal GNAS antibody

GNAS antibody

70R-13520 100 ul
EUR 550
Description: Affinity purified Rabbit polyclonal GNAS antibody

GNAS antibody

70R-1643 100 ug
EUR 407
Description: Rabbit polyclonal GNAS antibody raised against the N terminal of GNAS

GNAS antibody

70R-1651 100 ug
EUR 407
Description: Rabbit polyclonal GNAS antibody raised against the C terminal of GNAS

GNAS antibody

70R-1652 100 ug
EUR 407
Description: Rabbit polyclonal GNAS antibody raised against the N terminal of GNAS

GNAS antibody

70R-21619 50 ul
EUR 289
Description: Rabbit polyclonal GNAS antibody

GNAS antibody

70R-5756 50 ug
EUR 467
Description: Rabbit polyclonal GNAS antibody raised against the N terminal of GNAS

GNAS Antibody

1-CSB-PA009596GA01HU
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  • 150ul
  • 50ul
Description: A polyclonal antibody against GNAS. Recognizes GNAS from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB

GNAS Antibody

1-CSB-PA247539
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  • 100ul
  • 50ul
Description: A polyclonal antibody against GNAS. Recognizes GNAS from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:5000, WB:1:200-1:1000, IHC:1:25-1:100

GNAS Antibody

1-CSB-PA307619LA01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against GNAS. Recognizes GNAS from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500

GNAS Antibody

1-CSB-PA352389LA01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against GNAS. Recognizes GNAS from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200

GNAS Antibody

1-CSB-PA147947
  • Ask for price
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  • 100ul
  • 50ul
Description: A polyclonal antibody against GNAS. Recognizes GNAS from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:25-1:100

GNAS Antibody

ABD7068 100ug
EUR 325

GNAS Antibody

ABD7388 100ug
EUR 325

GNAS Antibody

F40026-0.4ML 0.4 ml
EUR 322.15
Description: Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The Gs protein is involved in hormonal regulation of adenylate cyclase: it activates the cyclase in response to beta-adrenergic stimuli. Alternative splicing of downstream exons of the GNAS gene is observed, which results in different forms of the stimulatory G protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants have been found for this gene, but the full-length nature and/or biological validity of some variants have not been determined. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.

GNAS Antibody

GWB-60D8A8 0.1 mg Ask for price

GNAS Antibody

GWB-DFA7EE 0.1 mg Ask for price

GNAS Antibody

GWB-MN943G 100ug Ask for price

GNAS Antibody

GWB-MP242H 50ug Ask for price

GNAS Antibody

R35181-100UG 100 ug
EUR 339.15
Description: Additional name(s) for this target protein: Guanine nucleotide binding protein alpha stimulating activity polypeptide 1

GNAS Antibody

MBS7107179-005mg 0.05mg
EUR 190

GNAS Antibody

MBS7107179-01mg 0.1mg
EUR 270

GNAS Antibody

MBS7107179-5x01mg 5x0.1mg
EUR 1205

GNAS Antibody

MBS7123704-005mL 0.05mL
EUR 190

GNAS Antibody

MBS7123704-01mL 0.1mL
EUR 270

GNAS Antibody

MBS7123704-5x01mL 5x0.1mL
EUR 1205

GNAS Antibody

MBS7123705-005mL 0.05mL
EUR 190

GNAS Antibody

MBS7123705-01mL 0.1mL
EUR 270

GNAS Antibody

MBS7123705-5x01mL 5x0.1mL
EUR 1205

GNAS Antibody

MBS7006626-005mg 0.05mg
EUR 190

GNAS Antibody

MBS7006626-01mg 0.1mg
EUR 270

GNAS Antibody

MBS7006626-5x01mg 5x0.1mg
EUR 1205

GNAS antibody

MBS9412789-005mL 0.05mL
EUR 300

GNAS antibody

MBS9412789-01mL 0.1mL
EUR 390

GNAS antibody

MBS9412789-5x01mL 5x0.1mL
EUR 1610

GNAS Antibody

MBS9413173-005mL 0.05mL
EUR 300

GNAS Antibody

MBS9413173-01mL 0.1mL
EUR 390

GNAS Antibody

MBS9413173-5x01mL 5x0.1mL
EUR 1610

GNAS Antibody

MBS8516510-01mg 0.1mg
EUR 305

GNAS Antibody

MBS8516510-01mLAF405L 0.1mL(AF405L)
EUR 465

GNAS Antibody

MBS8516510-01mLAF405S 0.1mL(AF405S)
EUR 465

GNAS Antibody

MBS8516510-01mLAF610 0.1mL(AF610)
EUR 465

GNAS Antibody

MBS8516510-01mLAF635 0.1mL(AF635)
EUR 465

GNAS Antibody

MBS8516821-01mg 0.1mg
EUR 305

GNAS Antibody

MBS8516821-01mLAF405L 0.1mL(AF405L)
EUR 465

GNAS Antibody

MBS8516821-01mLAF405S 0.1mL(AF405S)
EUR 465

GNAS Antibody

MBS8516821-01mLAF610 0.1mL(AF610)
EUR 465

GNAS Antibody

MBS8516821-01mLAF635 0.1mL(AF635)
EUR 465

GNAS Antibody

MBS8500866-01mg 0.1mg
EUR 325

GNAS Antibody

MBS8500866-01mLAF405L 0.1mL(AF405L)
EUR 565

GNAS Antibody

MBS8500866-01mLAF405S 0.1mL(AF405S)
EUR 565

GNAS Antibody

MBS8500866-01mLAF610 0.1mL(AF610)
EUR 565

GNAS Antibody

MBS8500866-01mLAF635 0.1mL(AF635)
EUR 565

GNAS Antibody

MBS8503174-01mL 0.1mL
EUR 325

GNAS Antibody

MBS8503174-01mLAF405L 0.1mL(AF405L)
EUR 565

GNAS Antibody

MBS8503174-01mLAF405S 0.1mL(AF405S)
EUR 565

GNAS Antibody

MBS8503174-01mLAF610 0.1mL(AF610)
EUR 565

GNAS Antibody

MBS8503174-01mLAF635 0.1mL(AF635)
EUR 565

GNAS Antibody

MBS8504502-01mg 0.1mg
EUR 325

GNAS Antibody

MBS8504502-01mLAF405L 0.1mL(AF405L)
EUR 565

GNAS Antibody

MBS8504502-01mLAF405S 0.1mL(AF405S)
EUR 565

GNAS Antibody

MBS8504502-01mLAF610 0.1mL(AF610)
EUR 565

GNAS Antibody

MBS8504502-01mLAF635 0.1mL(AF635)
EUR 565

GNAS Antibody

MBS2520647-006mL 0.06mL
EUR 190

GNAS Antibody

MBS2520647-012mL 0.12mL
EUR 265

GNAS Antibody

MBS2520647-02mL 0.2mL
EUR 415

GNAS Antibody

MBS2520647-5x02mL 5x0.2mL
EUR 1835

GNAS Antibody

MBS5312039-01mL 0.1mL
EUR 1070

GNAS Antibody

MBS5312039-5x01mL 5x0.1mL
EUR 4655

GNAS Antibody

MBS5312409-01mL 0.1mL
EUR 1070

GNAS Antibody

MBS5312409-5x01mL 5x0.1mL
EUR 4655

GNAS Antibody

MBS9607721-01mL 0.1mL
EUR 260

GNAS Antibody

MBS9607721-02mL 0.2mL
EUR 305

GNAS Antibody

MBS9607721-5x02mL 5x0.2mL
EUR 1220

GNAS Antibody

MBS9608025-01mL 0.1mL
EUR 260

GNAS Antibody

MBS9608025-02mL 0.2mL
EUR 305

GNAS Antibody

MBS9608025-5x02mL 5x0.2mL
EUR 1220

GNAS Complex Locus (GNAS) Antibody (HRP)

20-abx337231
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

GNAS Complex Locus (GNAS) Antibody (FITC)

20-abx337232
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  • Ask for price
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

GNAS Complex Locus (GNAS) Antibody (Biotin)

20-abx337233
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

anti- GNAS antibody

FNab03536 100µg
EUR 606.3
Description: Antibody raised against GNAS

GNAS Antibody (HRP)

20-abx306647
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  • Ask for price
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

GNAS Antibody (FITC)

20-abx306648
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  • Ask for price
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

NESP55,GNAS Antibody

abx235663-100ug 100 ug
EUR 577.2

GNAS Antibody (Biotin)

20-abx306649
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  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

NESP55,GNAS Antibody

abx235663-100g 100 µg
EUR 350

GNAS Antibody (Center)

MBS9214398-008mL 0.08mL
EUR 210

GNAS Antibody (Center)

MBS9214398-04mL 0.4mL
EUR 430

GNAS Antibody (Center)

MBS9214398-5x04mL 5x0.4mL
EUR 1910

GNAS complex locus (GNAS) polyclonal antibody

ABP-PAB-11314 100 ug Ask for price

GNAS Antibody (Ascites)

MBS9200490-01mL 0.1mL
EUR 430

GNAS Antibody (Ascites)

MBS9200490-5x01mL 5x0.1mL
EUR 1910

GNAS Antibody (C-term)

MBS9205942-008mL 0.08mL
EUR 210

GNAS Antibody (C-term)

MBS9205942-04mL 0.4mL
EUR 430

GNAS Antibody (C-term)

MBS9205942-5x04mL 5x0.4mL
EUR 1910

GNAS Antibody (N-Term)

MBS9215820-005mL 0.05mL
EUR 210

GNAS Antibody (N-Term)

MBS9215820-02mL 0.2mL
EUR 430

GNAS Antibody (N-Term)

MBS9215820-5x02mL 5x0.2mL
EUR 1910

GNAS Antibody (C-term)

MBS9203183-008mL 0.08mL
EUR 210

GNAS Antibody (C-term)

MBS9203183-04mL 0.4mL
EUR 430

GNAS Antibody (C-term)

MBS9203183-5x04mL 5x0.4mL
EUR 1910

anti- NESP55,GNAS antibody

FNab05663 100µg
EUR 606.3
Description: Antibody raised against NESP55,GNAS

Anti-NESP55,GNAS antibody

PAab05663 100ug
EUR 245

GNAS Antibody, HRP conjugated

1-CSB-PA307619LB01HU
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  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against GNAS. Recognizes GNAS from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

GNAS Antibody, HRP conjugated

1-CSB-PA352389LB01HU
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  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against GNAS. Recognizes GNAS from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

GNAS Antibody, HRP conjugated

MBS7107180-005mg 0.05mg
EUR 190

GNAS Antibody, HRP conjugated

MBS7107180-01mg 0.1mg
EUR 270

GNAS Antibody, HRP conjugated

MBS7107180-5x01mg 5x0.1mg
EUR 1205

GNAS Antibody; HRP conjugated

MBS7003350-005mg 0.05mg
EUR 190

GNAS Antibody; HRP conjugated

MBS7003350-01mg 0.1mg
EUR 270

GNAS Antibody; HRP conjugated

MBS7003350-5x01mg 5x0.1mg
EUR 1205

GNAS Antibody, FITC conjugated

1-CSB-PA307619LC01HU
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  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against GNAS. Recognizes GNAS from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

GNAS Antibody, FITC conjugated

1-CSB-PA352389LC01HU
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  • Ask for price
  • 100ug
  • 50ug
Description: A polyclonal antibody against GNAS. Recognizes GNAS from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

GNAS Antibody, FITC conjugated

MBS7107181-005mg 0.05mg
EUR 190

GNAS Antibody, FITC conjugated

MBS7107181-01mg 0.1mg
EUR 270

GNAS Antibody, FITC conjugated

MBS7107181-5x01mg 5x0.1mg
EUR 1205

GNAS Antibody; FITC conjugated

MBS7004175-005mg 0.05mg
EUR 190

GNAS Antibody; FITC conjugated

MBS7004175-01mg 0.1mg
EUR 270

GNAS Antibody; FITC conjugated

MBS7004175-5x01mg 5x0.1mg
EUR 1205

OAAN01353-50UL - GNAS Antibody

OAAN01353-50UL 50ul
EUR 149

Polyclonal GNAS Antibody (Center)

APR05815G 0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GNAS (Center). This antibody is tested and proven to work in the following applications:

GNAS Antibody, Biotin conjugated

1-CSB-PA307619LD01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against GNAS. Recognizes GNAS from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

GNAS Antibody, Biotin conjugated

1-CSB-PA352389LD01HU
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  • 100ug
  • 50ug
Description: A polyclonal antibody against GNAS. Recognizes GNAS from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

OAAN01353-100UL - GNAS Antibody

OAAN01353-100UL 100ul
EUR 249

OAAN01353-200UL - GNAS Antibody

OAAN01353-200UL 200ul
EUR 389

OAAJ07854-100UL - GNAS Antibody

OAAJ07854-100UL 100uL
EUR 319

OAGA00440-100UL - GNAS antibody

OAGA00440-100UL 100ul
EUR 369

OAGA00997-100UL - GNAS antibody

OAGA00997-100UL 100ul
EUR 369

GNAS Antibody, Biotin conjugated

MBS7107182-005mg 0.05mg
EUR 190

GNAS Antibody, Biotin conjugated

MBS7107182-01mg 0.1mg
EUR 270

GNAS Antibody, Biotin conjugated

MBS7107182-5x01mg 5x0.1mg
EUR 1205

GNAS Antibody; Biotin conjugated

MBS969903-005mg 0.05mg
EUR 190

GNAS Antibody; Biotin conjugated

MBS969903-01mg 0.1mg
EUR 270

GNAS Antibody; Biotin conjugated

MBS969903-5x01mg 5x0.1mg
EUR 1205

Rabbit Polyclonal antibody to GNAS (GNAS complex locus)

TA308888 100 µl Ask for price

Rabbit Polyclonal antibody to GNAS (GNAS complex locus)

TA308889 100 µl Ask for price

GNAS Polyclonal Antibody

A64422
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  • Ask for price
  • 100 µg
  • 20 ul
  • 50 ul
  • 100 ul

GNAS Polyclonal Antibody

A68803
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  • Ask for price
  • Ask for price
  • Ask for price
  • 100 ?g
  • 20 ul
  • 50 ul
  • 100 ul

GNAS Conjugated Antibody

C32899 100ul
EUR 476.4

GNAS Polyclonal Antibody

E-AB-14088-120uL 120uL
EUR 240
Description: Unconjugated

GNAS Polyclonal Antibody

E-AB-14088-200uL 200uL
EUR 399
Description: Unconjugated

GNAS Polyclonal Antibody

E-AB-14088-20uL 20uL
EUR 73
Description: Unconjugated

GNAS Polyclonal Antibody

E-AB-14088-60uL 60uL
EUR 143
Description: Unconjugated

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